phytopathogenic strain atcc 43348 Search Results


phytopathogenic strain atcc 43348  (ATCC)
91
ATCC phytopathogenic strain atcc 43348
Phytopathogenic Strain Atcc 43348, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
phytopathogenic strain atcc 43348 - by Bioz Stars, 2026-02
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anti human ve cadherin antibody  (Bio-Techne corporation)
99
Bio-Techne corporation anti human ve cadherin antibody
In vitro C5a-induced degranulated neutrophil contents degrade <t>VE-cadherin,</t> generating intercellular gaps. A) C5a-induced degranulated neutrophil contents (30 ng of supernatant) were incubated with HUVEC monolayers. Loss of VE-cadherin (asterisks, left lower panel) and appearance of intercellular gaps (right lower panel) at time 0 (Media) and 30 min. VE-cadherin (red), F-actin (green), DAPI (blue). Scale bar = 30 μm. B) Quantification of intercellular gap formation over time, as detailed in Methods. <t>C)</t> <t>Incubation</t> of C5a-induced degranulated neutrophil supernatant with HUVECs led to VE-cadherin degradation. D) Neutrophil protease inhibitors, specifically NE inhibitor (NEi) or the combination of CGi, NEi, PR3i and MMPi (x4i), blocked VE-cadherin degradation. Actin served as control for protein loading. Statistical analysis was performed using one-way ANOVA.
Anti Human Ve Cadherin Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human ve cadherin antibody/product/Bio-Techne corporation
Average 99 stars, based on 1 article reviews
anti human ve cadherin antibody - by Bioz Stars, 2026-02
99/100 stars
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Image Search Results


In vitro C5a-induced degranulated neutrophil contents degrade VE-cadherin, generating intercellular gaps. A) C5a-induced degranulated neutrophil contents (30 ng of supernatant) were incubated with HUVEC monolayers. Loss of VE-cadherin (asterisks, left lower panel) and appearance of intercellular gaps (right lower panel) at time 0 (Media) and 30 min. VE-cadherin (red), F-actin (green), DAPI (blue). Scale bar = 30 μm. B) Quantification of intercellular gap formation over time, as detailed in Methods. C) Incubation of C5a-induced degranulated neutrophil supernatant with HUVECs led to VE-cadherin degradation. D) Neutrophil protease inhibitors, specifically NE inhibitor (NEi) or the combination of CGi, NEi, PR3i and MMPi (x4i), blocked VE-cadherin degradation. Actin served as control for protein loading. Statistical analysis was performed using one-way ANOVA.

Journal: Molecular immunology

Article Title: Complement activation on neutrophils initiates endothelial adhesion and extravasation

doi: 10.1016/j.molimm.2019.09.011

Figure Lengend Snippet: In vitro C5a-induced degranulated neutrophil contents degrade VE-cadherin, generating intercellular gaps. A) C5a-induced degranulated neutrophil contents (30 ng of supernatant) were incubated with HUVEC monolayers. Loss of VE-cadherin (asterisks, left lower panel) and appearance of intercellular gaps (right lower panel) at time 0 (Media) and 30 min. VE-cadherin (red), F-actin (green), DAPI (blue). Scale bar = 30 μm. B) Quantification of intercellular gap formation over time, as detailed in Methods. C) Incubation of C5a-induced degranulated neutrophil supernatant with HUVECs led to VE-cadherin degradation. D) Neutrophil protease inhibitors, specifically NE inhibitor (NEi) or the combination of CGi, NEi, PR3i and MMPi (x4i), blocked VE-cadherin degradation. Actin served as control for protein loading. Statistical analysis was performed using one-way ANOVA.

Article Snippet: For immunofluorescence the chamber slides were blocked with 0.3% Triton X-100 (Thermo Fisher Scientific, Waltham, MA) with the addition of 10% goat serum (Sigma, Saint Louis MO) for 1 h at RT followed by incubation with anti-human VE-Cadherin antibody (1:100 dilution, cat# MAB9381, Bio-Techne Corporation, Minneapolis, MN) in a dilution buffer (PBS with 1% BSA, 1% goat serum and 0.3% Triton X-100) for 48-72 h at 4°C.

Techniques: In Vitro, Incubation